PHMcc partially co-migrated with a Golgi marker during subcellular fractionation and partially co-localized with Ccc2 based on immunofluorescence. Although ccc2 and atx1 mutant yeast produce normal levels of PHMcc protein, it lacks catalytic activity.
The peptidylglycine alpha-amidating enzyme catalyzes a reaction that transforms a carboxyl-terminal glycine-extended precursor into a carboxyl-terminal alpha-amidated peptide. We purified an alpha-amidating enzyme from equine serum by simplified steps including substrate affinity chromatography.
Peptidyl-glycine alpha-amidating monooxygenase is an enzyme that is required for the biosynthesis of many signaling peptides. This transformation is achieved by conversion of a prohormone to the corresponding amide C O NH 2.
In vertebrates, peptidylglycine alpha-amidating monooxygenase PAM is a multifunctional protein found in secretory granules. The protein contains two enzymes, peptidylglycine alpha-hydroxylating monooxygenase PHM and peptidyl-alpha-hydroxyglycine alpha-amidating lyase PAL , that act sequentially to catalyse the alpha-amidation of neuroendocrine peptides [ PMID:
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